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SHOTGUN LIPIDOMICS
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This is a direct infusion-tandem mass spectrometry method utilizing a differential mobility system. This assay is performed on the Sciex 5500 Triple-Quadrupole (QQQ) and Sciex 6500+ Triple-Quadrupple (QQQ) with a Shimadzu auto-sampler configured for direct infusion experiments, SelexION ion mobility device, and Shimadzu LC. This assay is built on the Sciex Lipidyzer Platform. We use our in-house data analysis workflow and a combination of 74 lipid standards sourced from Avanti Polar Lipids to provide accurate, quantitative measurements of over 1400 lipid species across 17 lipid subclasses within a biological sample. Download the excel file for a complete list of lipid species measured in this targeted assay.
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LIPID COMPOSITIONAL ANALYSIS
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This assay quantitatively measures the fatty acid and cholesterol composition of a biological sample. In situ derivatization breaks up complex esterified lipids into constituent fatty acids and cholesterol, which can be measured by gas chromatography/mass spectrometer. The assay is performed on an Agilent 7890B/5977A GC-MS, and is compatible with stable isotope labeling. Data analysis utilizes the mathematical modeling-based isotopic spectral analysis (ISA) method to provide estimations of flux and lipid synthesis rates, as well as calculating the contribution of carbon sources to the acetyl-CoA pool.
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